Invitrogen pet manual

pETDuet™-1 DNA - Novagen pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites, each of which is preceded by a T7 promoter, lac operator and ribosome binding sites. - Find MSDS or SDS, a COA, data sheets and more information. With the Champion™ pET Directional TOPO® vectors you can expect highest-level protein production. Figure 2 shows expression of the lac Z gene in Champion™ pET Directional TOPO® vectors. Figure 3 demonstrates efficient cleavage using TEV protease of the N-terminal tag of a β-galactosidase fusion protein expressed from pET/D-TOPO®. pET SUMO TA Cloning® Reagents The following reagents are included with the pET SUMO vector (Box 1). Note that the user must supply Taq polymerase. Store Box 1 at °C. Item Concentration Amount pET SUMO vector, linearized 25 ng/ μl in: 10 mM Tris-HCl, pH 1 mM EDTA, pH 5 x 10 l 10X PCR Buffer mM Tris-HCl, pH (at 42°C) mM KClFile Size: KB.

pET SUMO TA Cloning® Reagents The following reagents are included with the pET SUMO vector (Box 1). Note that the user must supply Taq polymerase. Store Box 1 at °C. Item Concentration Amount pET SUMO vector, linearized 25 ng/ μl in: 10 mM Tris-HCl, pH 1 mM EDTA, pH 5 x 10 l 10X PCR Buffer mM Tris-HCl, pH (at 42°C) mM KCl. pET System Manual TB 8th Edition 02/99 Novagen 3 United States Canada Orders: Technical Service: I. About the System A. Description The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Target genes are cloned in pET plasmids under control of strong. This second printing of the 10 th edition of the pET Manual was published May, Novagen is continually expanding and upgrading the pET System. Please check the Novagen website, www.doorway.ru, for updated pET System Manual information. Table of Contents Table of Contents 1 I. About the System 4 A. Description 4.

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